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90
Procell Inc ttt/gf pituitary tumor cell line
a CCK-8 cell viability assay of GH3, <t>AtT-20,</t> and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).
Ttt/Gf Pituitary Tumor Cell Line, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pituitary+tumor+cell+line+ttt/rat+pituitary+tumor+gh3+cells/pmc12122724-361-1-5
Average 90 stars, based on 1 article reviews
ttt/gf pituitary tumor cell line - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Procell Inc pituitary tumor cell line ttt/gf
a CCK-8 cell viability assay of GH3, <t>AtT-20,</t> and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).
Pituitary Tumor Cell Line Ttt/Gf, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pituitary+tumor+cell+line+ttt/rat+pituitary+tumor+gh3+cells/pm40442104-364-1-5
Average 90 stars, based on 1 article reviews
pituitary tumor cell line ttt/gf - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


a CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).

Journal: Nature Communications

Article Title: Single-cell and spatial transcriptome analyses reveal tumor heterogeneity and immune remodeling involved in pituitary neuroendocrine tumor progression

doi: 10.1038/s41467-025-60028-5

Figure Lengend Snippet: a CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).

Article Snippet: The pituitary tumor cell line TtT/GF(Procell, CL-0561) is cultured in DMEM/F12 medium supplemented with 10% horse serum, 2.5% FBS, 2 ng/ml basic fibroblast growth factor (bFGF), and 1% penicillin-streptomycin (Procell, CM-0561).

Techniques: CCK-8 Assay, Viability Assay, Wound Healing Assay, Transwell Invasion Assay, Immunofluorescence, Cell Culture, Derivative Assay, Cell Viability Assay

a Western blot analysis of ITGB1 expression in GH3, AtT-20, and TtT/GF cell lines. b Semi-quantitative analysis of ITGB1 in GH3, AtT-20, and TtT/GF cell lines ( n = 3 biological replicates). c CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). d Transwell invasion assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). e Quantitative analysis of Transwell invasion assay in GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). Scale bar, 100 µm. Two-sided unpaired t -test was performed for ( b − e ). Data are presented as mean values +/- SD for ( b − e ). Source data are provided as a Source Data file for ( a − e ).

Journal: Nature Communications

Article Title: Single-cell and spatial transcriptome analyses reveal tumor heterogeneity and immune remodeling involved in pituitary neuroendocrine tumor progression

doi: 10.1038/s41467-025-60028-5

Figure Lengend Snippet: a Western blot analysis of ITGB1 expression in GH3, AtT-20, and TtT/GF cell lines. b Semi-quantitative analysis of ITGB1 in GH3, AtT-20, and TtT/GF cell lines ( n = 3 biological replicates). c CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). d Transwell invasion assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). e Quantitative analysis of Transwell invasion assay in GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). Scale bar, 100 µm. Two-sided unpaired t -test was performed for ( b − e ). Data are presented as mean values +/- SD for ( b − e ). Source data are provided as a Source Data file for ( a − e ).

Article Snippet: The pituitary tumor cell line TtT/GF(Procell, CL-0561) is cultured in DMEM/F12 medium supplemented with 10% horse serum, 2.5% FBS, 2 ng/ml basic fibroblast growth factor (bFGF), and 1% penicillin-streptomycin (Procell, CM-0561).

Techniques: Western Blot, Expressing, CCK-8 Assay, Viability Assay, Transwell Invasion Assay